Anti-NFκB-p65 Antibody

This rabbit polyclonal antibody targets the p65 subunit (also known as RelA) of the NF-κB transcription factor complex, a central regulator of inflammatory signaling, immune responses, and cell survival pathways. Produced in rabbit and supplied as an unconjugated reagent in a 100 µl volume, it is validated for western blot and ELISA workflows.

Cross-reactivity has been confirmed against human, mouse, and rat samples, supporting its use across immunology, oncology, and inflammation research models. Investigators commonly apply this reagent to study TNF-α–driven signaling cascades, IκB kinase activity, and downstream transcriptional targets such as COX-2 and IL-6 in cell lysates.

As a polyclonal reagent, the antibody recognizes multiple epitopes on the p65 subunit, which can improve detection sensitivity in western blot applications where post-translational modifications or splice variants may otherwise reduce recognition by monoclonal clones. Provided by Antibodies.com as a primary antibody intended for research use only, the reagent supports investigations of canonical NF-κB activation, nuclear translocation, and DNA-binding activity assays.