Anti-NFκB p65 Antibody

This unconjugated mouse monoclonal antibody from Antibodies.com recognizes the p65 (RelA) subunit of NFκB, the master transcription factor complex that governs inflammatory cytokine expression, immune cell activation, and anti-apoptotic gene programs. The reagent is supplied as a 100 µl liquid aliquot and is validated for Western blot, immunofluorescence, and immunoprecipitation workflows on human, rat, and mouse samples.

p65 immunodetection is a workhorse assay in signal-transduction research, used to track canonical pathway activation downstream of TNF-α, IL-1β, and Toll-like receptor signaling, and to monitor IκBα phosphorylation and subsequent nuclear translocation of the p65/p50 heterodimer. Because the antibody cross-reacts with three commonly studied mammalian species, it supports comparative studies that pair primary rodent tissue or disease-model extracts with patient-derived human cell lines, including work on chronic inflammation, tumor progression, autoimmune disease, and innate immune responses.

Recommended working dilutions, suggested positive controls (such as TNF-α-stimulated HeLa or RAW 264.7 lysates), and counterstain guidance for visualizing p65 nuclear localization by immunofluorescence are detailed on the Antibodies.com product datasheet.