Anti-NFκB p65 Antibody

Offered through Antibodies.com as a primary antibody reagent for transcription factor research, this mouse monoclonal reagent targets the NFκB p65 subunit (RELA) and is supplied as 100 µl of an affinity-purified, unconjugated IgG1 at 1.0 mg/mL. The antibody specifically detects endogenous p65 protein and is validated for Western blotting (WB) and immunohistochemistry (IHC) applications, with broad reactivity confirmed against human, mouse and rat samples. IHC staining of mouse hippocampus tissue has been demonstrated with the antibody diluted at 1:200, supporting its use in neuroscience and inflammation studies. The formulation is provided in phosphate buffered saline (without Mg²⁺ and Ca²⁺) at pH 7.4, supplemented with 150 mM NaCl, 0.02% sodium azide and 50% glycerol as a cryoprotectant, and the product is shipped at 4°C; upon arrival it should be aliquoted and stored at -20°C, with repeated freeze/thaw cycles avoided to preserve activity. As a Mouse IgG1 isotype monoclonal with no fluorescent or enzymatic label attached, the reagent is well suited to chromogenic IHC detection and standard immunoblotting workflows, while also being compatible with secondary antibody-based detection systems chosen by the end user. The conjugate status (unconjugated) and clonality (monoclonal) together provide reproducible lot-to-lot specificity for NFκB p65, and the broad species cross-reactivity makes this Antibodies.com reagent a flexible choice for comparative studies across human cell lines and rodent tissue models.