Anti-NFκB-p65 Antibody

This polyclonal antibody from Antibodies.com is raised in rabbit against the p65 subunit of NF-kappa B (also called RelA), a transcription factor central to inflammatory signaling, immune responses, and cell-survival pathways. Supplied as an unconjugated reagent in a 100 ul vial, the antibody is validated for western blot, immunohistochemistry, and ELISA applications, and it recognizes the target in human, mouse, and rat samples. Researchers working on cytokine-driven signaling, tumor progression, or autoimmune mechanisms rely on p65-specific detection to follow pathway activation: under resting conditions the p65 subunit is held in the cytoplasm by I-kappa B inhibitors, and upon stimulation it translocates to the nucleus, where it can be tracked in fixed tissue sections or cell lysates. The polyclonal format increases the likelihood of recognizing multiple epitopes on the approximately 65 kDa RelA protein, which is useful when working with samples that may carry post-translational modifications such as phosphorylation at Ser536. For handling, aliquot upon first use and store the vial frozen at -20 degrees C in single-use portions to preserve activity across repeated experiments, keeping a working stock at 4 degrees C for short-term access. Provided as a 100 ul liquid reagent for research use only.