Anti-NFκB-p65 Antibody

NFκB-p65 (also known as RelA) is a key transcription factor subunit of the canonical NF-κB signaling pathway, where it drives inflammatory responses, immune regulation, and cell survival through IκBα degradation and subsequent nuclear translocation. This polyclonal reagent from Antibodies.com is raised in rabbit hosts, supplied as an unconjugated preparation in a 100 µL vial, and validated for western blotting, immunohistochemistry, and ELISA applications. It detects the p65 subunit across human, mouse, and rat samples, supporting cross-species studies of cytokine-driven signaling, tumor biology, and innate immunity. The polyclonal format recognizes multiple epitopes on the RelA protein, which is useful when probing full-length and truncated variants, post-translational modifications, or cleaved fragments generated during apoptosis. Researchers investigating TNF-α, LPS, or oxidative-stress–mediated activation commonly use this reagent to track p65 translocation between cytoplasmic and nuclear fractions and to assess basal versus stimulated pathway activity in cultured cells and tissue sections.