Anti-NFκB-p65 Antibody

The p65 subunit (RelA) anchors the canonical NF-κB transcription factor complex and drives expression of genes governing inflammation, immune cell activation, apoptosis, and proliferation. This unconjugated rabbit polyclonal antibody from Antibodies.com targets the p65 subunit and is supplied as 100 µl of liquid reagent, suitable for western blot, immunohistochemistry, and ELISA workflows across human, mouse, and rat sample types.

The reagent detects endogenous levels of total p65 and is widely applied in studies of cytokine-driven signaling, tumor progression pathways, and inflammatory disease models. Because p65 translocates from cytoplasm to nucleus upon pathway activation, the antibody is frequently paired with subcellular fractionation protocols, or with phospho-specific antibodies for IκBα, to distinguish resting from activated signaling states. Cross-reactivity with mouse and rat permits comparative work in rodent disease models alongside human tissue specimens, supporting translational research in oncology, neurodegeneration, and autoimmunity.

The unconjugated format allows flexible pairing with HRP, biotin, or fluorophore secondary reagents depending on the detection chemistry required by each assay. Aliquoting the vial upon receipt and storage under standard refrigerated conditions helps preserve polyclonal antibody activity across repeated experiments.