Anti-NFκB-p65 Antibody

This polyclonal antibody from Antibodies.com targets the p65 subunit (RelA) of NF-κB, a transcription factor that drives inflammatory signaling, immune cell activation, and cellular stress responses. Raised in rabbit and supplied as an unconjugated reagent in 100 µl, the reagent is compatible with standard immunodetection workflows.

Validated applications include Western blot for tracking p65 expression and phosphorylation status across human, mouse, and rat samples, immunohistochemistry for visualizing nuclear translocation in fixed tissue sections, and ELISA-based quantification. The unconjugated format allows researchers to couple the reagent to their preferred reporter enzyme or fluorophore depending on experimental design.

NF-κB-p65 plays a central role in canonical NF-κB signaling. Following stimulation by cytokines such as TNF-α or IL-1β, IκB is phosphorylated and degraded, freeing p65 to translocate to the nucleus and activate transcription of target genes involved in inflammation, cell survival, and proliferation. Aberrant p65 activity is implicated in chronic inflammatory disease, autoimmunity, and several cancer types, making this reagent a frequently studied tool in cell signaling and disease research.