Anti-NFκB-p65 Antibody

Rabbit polyclonal antibody raised against the NFκB-p65 transcription factor subunit (also known as RelA), supplied by Antibodies.com as an unconjugated primary antibody in a 100 µl vial. NFκB-p65 is a central mediator of canonical NF-κB signaling, translocating to the nucleus upon activation to drive expression of genes involved in inflammation, innate immunity, cell proliferation, and apoptosis resistance. Researchers use this reagent to detect endogenous p65 in human and mouse samples across western blot, immunohistochemistry, immunofluorescence, and ELISA workflows.

The polyclonal format increases the likelihood of recognizing multiple epitopes on the p65 protein, which is useful when probing full-length versus cleaved or post-translationally modified forms of the subunit in cell culture and tissue-based assays. Because the antibody is unconjugated, it can be paired with HRP, fluorophore, or biotin-labeled secondary reagents chosen for the detection modality of interest. Each laboratory should empirically determine optimal dilutions for its specific substrate and detection chemistry.

Storage and handling: aliquot upon receipt to limit freeze-thaw cycles and store refrigerated; protect from light when working with fluorescent secondaries. For research use only, not for diagnostic or therapeutic applications.