Anti-NFκB-p65 Antibody

The Antibodies.com rabbit polyclonal reagent targets the NFκB-p65 subunit (also called RelA), the canonical DNA-binding component of the NF-κB transcription complex that drives inflammatory gene expression, cytokine production, and cell-survival signaling. Raised in rabbit and supplied as an unconjugated 100 µl liquid aliquot, the antibody recognizes native and denatured p65 protein, supporting multiple readouts in fixed and unfixed samples.

Validated workflows include Western blot for total and phosphorylated p65 detection in cell lysates, immunohistochemistry on paraffin and frozen tissue sections, immunofluorescence for visualizing cytoplasmic-to-nuclear translocation following TNF-α or LPS stimulation, and ELISA-based quantitation of p65 in experimental samples. The reagent shows reactivity with Human, Mouse, and Monkey samples, useful for translational comparisons between rodent disease models and primate or human specimens.

Because the antibody is polyclonal, it binds several epitopes on the p65 molecule, which can improve tolerance to minor denaturation or partial proteolysis during sample preparation. The unconjugated format lets researchers pair it with HRP, fluorophore, or biotin secondary reagents of their choice. Refrigerate the vial in working aliquots to limit freeze-thaw cycles and preserve reagent activity through its stated shelf life.