Anti-Neutrophil Cytosol Factor 1 (Ab-304) Antibody

This Rabbit polyclonal antibody from Antibodies.com targets Neutrophil Cytosol Factor 1 (NCF1, also known as p47phox or NOXO2), a47 kDa cytosolic organizer of the NADPH oxidase complex. The reagent is raised against a synthetic non-phosphopeptide derived from human NCF1 surrounding serine 304 (R-S-S-I-R), enabling detection of total endogenous NCF1 protein rather than the phosphorylated form. It is supplied as 50 µL of affinity-purified antibody at 1.0 mg/mL in phosphate buffered saline (pH 7.4) containing 150 mM NaCl, 0.02% sodium azide and 50% glycerol, without magnesium or calcium. The host species is Rabbit (polyclonal IgG), the conjugate is unconjugated, and validated reactivity is limited to human samples. Recommended applications include Western blotting at 1:500–1:3000 and immunofluorescence (ICC/IF) at 1:100–1:500, making it well suited to studies of phagocyte oxidative burst, chronic granulomatous disease models, and signaling pathways involving the NOX2 complex. The product is intended for research use only and is not for diagnostic or therapeutic applications.