Anti-NeuN Antibody

Recombinant monoclonal antibody targeting NeuN (neuronal nuclei), also known as RBFOX3, a nuclear RNA-binding protein expressed in most post-mitotic neurons of the central nervous system. This unconjugated rabbit reagent is validated for Western blot, immunocytochemistry and immunofluorescence, immunohistochemistry, and flow cytometry workflows, with verified reactivity on human, mouse, and rat samples. Antibodies.com supplies this clone as 50 µl of recombinant rabbit monoclonal antibody in 1×TBS (pH 7.4) supplemented with 1% BSA and 40% glycerol, preserved with 0.05% sodium azide for routine refrigerated handling.

NeuN immunolabeling is widely adopted in neuroscience research to identify mature neurons in brain, spinal cord, and peripheral ganglion sections, separating neuronal populations from surrounding glia in development, injury, neurodegeneration, and disease model studies. The antibody binds a conserved epitope within the RBFOX3 protein, supporting cross-species comparisons between rodent tissue and human specimens in the same experimental series. Brief spin-down before opening recovers the full 50 µl volume, and the glycerol-based formulation tolerates storage at -20 °C after aliquoting, helping minimize freeze-thaw cycles and preserve binding activity across multiple staining sessions.