Anti-NET-5 Antibody

Researchers investigating post-synaptic adhesion molecules and leucine-rich repeat proteins frequently need a robust detection reagent for NET-5 in human and mouse samples. This polyclonal antibody from Antibodies.com was raised in rabbit and qualified for western blot and ELISA workflows, and the unconjugated preparation is supplied at 100 µl. Because polyclonal antibodies engage multiple epitopes on the same target, the reagent can be useful for resolving isoforms or post-translationally modified forms of NET-5 in lysates from cultured neurons, brain homogenates, or transfected cell lines.

Standard immunoblotting workflows with RIPA-type buffers and protease inhibitors recover the membrane-associated fraction effectively, and individual titration against a positive control such as mouse brain lysate is recommended to calibrate working concentration. For ELISA, the unconjugated format allows the end user to attach HRP, biotin, or a fluorophore of choice, which fits laboratories that maintain their own secondary antibody panels.

Aliquot on first use to limit freeze-thaw cycles, store refrigerated at 4 °C for short-term handling, and keep frozen for longer-term storage to preserve antibody activity. Handle with standard laboratory practice for protein reagents, and avoid repeated exposure to room temperature between uses. The rabbit host and polyclonal clonality make this antibody easy to pair with anti-rabbit secondaries commonly stocked across neuroscience and cell biology laboratories.