Anti-Nestin Antibody [7A3]

The Anti-Nestin Antibody [7A3] from Antibodies.com is a Rat IgG2b monoclonal reagent that recognizes the class VI intermediate filament protein Nestin, a well-characterized marker of neural stem and progenitor cells in the developing and adult mouse central nervous system. Supplied as a liquid in a 200 ug vial, this unconjugated primary antibody supports immunofluorescence (ICC/IF) and immunohistochemistry (IHC) labeling of Nestin-positive radial glia, neuroepithelial progenitors, and glioblastoma stem-like populations in cultured cells and fixed tissue sections. The clone 7A3 epitope maps within the conserved rod region of mouse Nestin, enabling reproducible detection across sections of embryonic and adult mouse brain used in neurogenesis, neural lineage-tracing, and tumor stem cell studies. For best stability, store aliquots frozen at -20 degrees C and limit freeze-thaw cycles; for active use, keep the vial refrigerated at 4 degrees C and protect from light. This reagent is supplied for research use only and pairs readily with anti-Rat IgG secondary conjugates for multiplex immunostaining workflows.