Anti-Nephrin Antibody [G17-H] - Identical to Abcam (ab136927) and Novus (NBP1-30137)

Antibodies.com offers a rabbit monoclonal antibody to Nephrin, raised against a peptide derived from the C-terminal sequence of the first Ig-like domain of human nephrin. The clone, designated G17-H, is an IgG isotype supplied unconjugated in liquid form.

This reagent is validated for Western blot (WB), ELISA, immunoprecipitation (IP) and immunocytochemistry (ICC), and it reacts with samples from human, mouse and rat. The antibody recognises an epitope located between Cys53 and Cys101 of nephrin.

It is formulated in Tris-buffered saline at pH 8.0, containing 10 mg/ml BSA and 0.05% sodium azide. The product is shipped at 4°C and should be aliquoted and stored at -20°C on receipt, with freeze/thaw cycles avoided. Recommended starting dilutions are WB: 1:2,000 and ELISA: 1:20,000–1:50,000.