Anti-NEK3 Antibody

Raised in rabbit, this unconjugated polyclonal reagent targets NEK3, a serine/threonine kinase of the NIMA-related family that participates in cell cycle progression, microtubule organization, and neuronal differentiation. NEK3 has also been investigated in HER2-positive breast cancer and in DNA damage response pathways, where altered expression has been linked to chemosensitivity profiles in tumor cell models.

The reagent is supplied as 100 µl at 1 mg/ml in a vial, formulated in PBS with 0.1% sodium azide and 50% glycerol, and is validated for Western blot, immunohistochemistry, and immunofluorescence workflows on human, mouse, and rat samples. Storage at -20 °C in working aliquots is recommended to limit freeze-thaw cycles, with the sodium azide acting as a preservative for short-term bench use.

Intended for research use only, this Antibodies.com reagent supports kinase signaling, mitotic entry, and neuronal cell biology studies through target verification on immunoblots, fixed tissue sections, and cultured cells. The unconjugated format leaves the choice of secondary label, fluorophore, or enzyme reporter open, and the polyclonal preparation enables recognition of multiple epitopes within NEK3.