Anti-NEK2 Antibody

Raised in rabbit against the NEK2 (NIMA-related kinase 2) protein, this polyclonal reagent from Antibodies.com supports studies of mitotic regulation, centrosome separation, and spindle assembly. NEK2 is a serine/threonine kinase that localizes to centrosomes and drives their disjunction at the G2/M transition, and it is frequently investigated in the context of centrosome amplification in breast, ovarian, and lung tumor models.

The antibody is supplied as 100 µl of a 1 mg/ml stock in PBS with 0.1% sodium azide and 50% glycerol, unconjugated for maximum flexibility in downstream labeling. It has been validated for western blot (WB), immunohistochemistry (IHC), and immunofluorescence (IF) applications and shows reactivity with human, mouse, and rat samples, making it useful across comparative cell cycle and oncology workflows.

Typical uses include tracking endogenous NEK2 levels in synchronized cell populations, probing centrosome maturation by IF in dividing cells, and examining NEK2 expression patterns in tumor tissue sections by IHC. Short-term storage at 4 °C is appropriate for working stocks, with aliquoting recommended to limit freeze-thaw cycles and preserve antibody titer during long-term storage at -20 °C.