Anti-NEK2 Antibody

Raised against NIMA-related kinase 2 in rabbit hosts, this polyclonal IgG reagent from Antibodies.com targets a centrosome-localized serine/threonine kinase that orchestrates mitotic spindle assembly and centriole disengagement during cell division. Investigators focused on chromosome segregation fidelity and cell-cycle checkpoint control use it to monitor endogenous NEK2 protein levels in cultured human and murine cell lines and in fixed tumor tissue sections.

Provided as 50 µl of unconjugated antiserum, the antibody supports three orthogonal readouts: Western blot for total expression and post-translational modification surveys, immunohistochemistry on formalin-fixed paraffin-embedded tissue for spatial distribution, and immunofluorescence for subcellular localization within the centrosomal compartment. The polyclonal format broadens epitope coverage across the full-length kinase, which can help probe splice variants or truncated isoforms reported in NEK2-overexpressing cancers, including breast carcinoma, colorectal adenocarcinoma, and diffuse large B-cell lymphoma.

Beyond standard expression profiling, the Antibodies.com reagent is useful in chromatin and cytoskeletal fractionation workflows used to study drug-induced centrosome amplification, mitotic catastrophe following kinase inhibition, and CRISPR-Cas9 loss-of-function phenotyping in transformed and non-transformed lines. Confirmed reactivity spans human and mouse orthologs, making it compatible with patient-derived samples and with standard murine xenograft or genetic knockout models.