Anti-NEIL2 Antibody

NEIL2 (Nei endonuclease VIII-like 2) is a DNA base excision repair glycosylase that excises oxidatively damaged bases such as 8-oxoguanine from genomic DNA. This rabbit polyclonal antibody from Antibodies.com is raised against the human NEIL2 protein and cross-reacts with rat samples, supporting comparative immunoblotting studies of repair enzyme expression across these two species.

The reagent is supplied as 100 µl of unconjugated antiserum at 1 mg/ml in phosphate buffered saline (PBS) containing 15 mM sodium azide as a preservative, at approximately pH 7.2. The polyclonal clonality provides multi-epitope recognition of the NEIL2 target, an advantage when probing expression across different sample preparations where reliance on a single linear epitope may give inconsistent results.

Research applications include profiling NEIL2 levels in cancer cell lines and tumor-derived samples, examining oxidative DNA damage responses in hepatic and neuronal tissues, and investigating repair enzyme regulation under genotoxic stress conditions. Because the antibody is unconjugated, researchers can pair it with standard anti-rabbit HRP- or fluorophore-labelled secondary reagents for chemiluminescent or fluorescent Western blot detection.

For handling, the azide-containing PBS formulation should be aliquoted upon arrival to limit repeated freeze-thaw exposure, with the bulk stored refrigerated between uses to preserve antibody activity. Working dilutions for Western blot should be determined empirically against the specific lysate type and detection system in use.