Anti-NEFL Antibody

Neurofilament light chain (NF-L) is a ~68 kDa class IV intermediate filament protein concentrated in neuronal axons, and Antibodies.com supplies a rabbit polyclonal antibody raised against NEFL for western blot workflows.

The unconjugated reagent is provided at 1 mg/ml in phosphate buffered saline with 0.05% sodium azide at approximately pH 7.2, with each vial containing 100 µl. Reactivity has been confirmed against human, mouse, and rat samples, supporting neuroscience and neuropathology projects.

NEFL has become a leading fluid biomarker of neuroaxonal injury in studies of amyotrophic lateral sclerosis, multiple sclerosis, Alzheimer's disease, and traumatic brain injury, so dependable antibody reagents are central to tracking cytoskeletal integrity in cell culture and tissue models.

The unconjugated format leaves the immunoglobulin available for direct western blot detection using HRP- or fluorophore-conjugated secondary antibodies. For storage, keep the vial refrigerated at 4 °C for short-term use or freeze aliquots at -20 °C for longer projects, avoiding repeated freeze-thaw cycles to preserve binding activity.

This polyclonal reagent is intended for research use only and enables visualization of endogenous NEFL expression across developmental, injury, and disease models.