Anti-NEDL2 Antibody

NEDL2 (also known as HECW2) is a HECT-family E3 ubiquitin-protein ligase involved in protein ubiquitination and turnover, and this rabbit polyclonal antibody from Antibodies.com is raised against it for research use. The unconjugated reagent is supplied as 100 µl of liquid and is validated for Western blot and ELISA workflows in human samples, with reactivity directed toward the endogenous ligase in cell lysates and recombinant targets.

Polyclonal sera from a rabbit host typically engage several regions of the immunogen, which can be useful when probing lower-abundance ligases, partially degraded proteins, or denatured lysates. The unconjugated format gives researchers flexibility to pair the antibody with HRP, biotin, fluorophore, or alkaline phosphatase secondary reagents to match their chosen detection platform.

For Western blot, the reagent can be used to follow NEDL2 expression across human cell lines and tissue samples, where it generally resolves as a high-molecular-weight band consistent with the full-length ligase. For ELISA, it supports direct or indirect coating strategies depending on the plate configuration. Storage at refrigerated temperatures with aliquoting upon first thaw is recommended to preserve activity across repeated experiments.