Anti-NEDD9 Antibody

Raised in rabbit and purified by affinity chromatography against the immunogen, this polyclonal reagent from Antibodies.com targets the NEDD9 protein (also known as HEF1/Cas-L), a member of the Cas family of adhesion docking molecules implicated in integrin signalling, cell migration and focal adhesion dynamics. The immunogen used to generate the antibody is a recombinant human NEDD9 protein, ensuring reactivity against endogenous human, mouse and rat NEDD9. The antibody is supplied as an unconjugated antiserum at 1.0 mg/mL in a 50 µL volume, formulated in phosphate-buffered saline at pH 7.4 containing 150 mM NaCl, 0.02% sodium azide and 50% glycerol as a cryoprotectant. It is shipped at 4 °C and should be aliquoted and stored long-term at -20 °C, with repeated freeze/thaw cycles avoided to preserve activity. Verified applications include Western blotting (WB), immunocytochemistry/immunofluorescence (ICC/IF) and immunohistochemistry (IHC), making it suitable for detection of total NEDD9 in cell lysates, cultured cells and tissue sections. Researchers studying focal adhesion turnover, cancer cell invasion, integrin-mediated signalling, or downstream FAK/Src pathway activation will find this reagent a reliable tool across multiple model systems. Each lot is validated to detect endogenous levels of total NEDD9, and the polyclonal nature of the antibody provides broader epitope coverage than monoclonal alternatives.