Anti-NEDD8 Antibody

Manufactured by Antibodies.com, this affinity-purified reagent is a rabbit polyclonal antibody raised against the ubiquitin-like modifier NEDD8, an 81-amino-acid protein that is conjugated to substrate lysines in a process analogous to ubiquitination. The immunogen is recombinant human NEDD8, and the resulting antibody recognizes the endogenous level of total NEDD8 protein across validated samples. It is supplied at 1.0 mg/mL in a 50 µl volume of phosphate-buffered saline (without Mg²⁺ and Ca²⁺), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide and 50% glycerol as a cryoprotectant. The host species is rabbit, the clonality is polyclonal, and the conjugate is unconjugated, making the reagent suitable for use with a wide range of secondary antibodies and detection chemistries. Verified applications include Western blot (WB), immunohistochemistry (IHC) and immunocytochemistry/immunofluorescence (ICC/IF), and reactivity has been confirmed in human, mouse and rat samples. For routine handling, the antibody is shipped at 4 °C; upon receipt it should be aliquoted and stored at –20 °C, with repeated freeze/thaw cycles avoided to preserve performance. As supplied by Antibodies.com, this polyclonal anti-NEDD8 reagent is intended for research use only and is not cleared for diagnostic or therapeutic applications. Researchers studying neddylation, CRL ligase regulation, or the crosstalk between NEDD8 and ubiquitin pathways will find this reagent a versatile addition to their antibody panels for cell biology, signalling and post-translational modification studies.