Anti-NEDD8 Antibody

Affinity-purified rabbit polyclonal IgG raised against a recombinant human NEDD8 immunogen, this unconjugated primary antibody recognizes the endogenous Neural precursor cell Expressed Developmentally Down-regulated 8 ubiquitin-like modifier (predicted ~10 kDa). Supplied by Antibodies.com as 50 µl of antiserum in PBS pH 7.30 with 0.02% sodium azide and 50% glycerol, the reagent is validated for Western blot at 1:500-1:2000 and immunohistochemistry at 1:50-1:200 across human, mouse, and rat samples, supporting studies of neddylation, Cullin-RING E3 ubiquitin ligase activation, cell cycle control, NF-κB signaling, and ubiquitin-proteasome cross-talk. The unconjugated IgG format pairs cleanly with HRP- or fluorophore-conjugated secondary reagents for chemiluminescent or fluorescence readouts. Vials are shipped at 4°C; aliquot upon arrival and store at -20°C in a frost-free freezer, avoiding repeated freeze-thaw cycles to preserve antibody activity. For research use only.