Anti-NEDD8 Antibody

Raised in rabbit and affinity purified against a recombinant human NEDD8 immunogen, this polyclonal IgG primary antibody from Antibodies.com is supplied as a 100 µL liquid aliquot for immunodetection of NEDD8, a 9 kDa ubiquitin-like modifier that covalently conjugates to cullin scaffold proteins. The unconjugated format supports western blot workflows at 1:500 to 1:2000 dilution and immunohistochemistry at 1:50 to 1:200, with reactivity confirmed against human, mouse, and rat samples.

The antibody recognizes both the free NEDD8 precursor and neddylated conjugates, enabling characterization of cullin-RING E3 ubiquitin ligase activation, cell cycle progression, and proteasomal substrate turnover. Common uses include deneddylation assays, studies of ubiquitin-proteasome pathway dynamics, and detection of cullin modification status in cancer and DNA damage response models.

Each vial contains 100 µL of antibody in phosphate buffered saline (pH 7.30) with 0.02% sodium azide and 50% glycerol as a cryoprotectant. Brief centrifugation before opening and refrigerated storage between uses preserves activity across repeated freeze-thaw cycles. The unconjugated reagent allows flexible pairing with HRP, biotin, or fluorophore-conjugated secondary reagents depending on experimental design. The product is intended for research use only.