Anti-NECAB3 Antibody

Manufactured by Antibodies.com, this rabbit polyclonal reagent is raised against the N-terminal EF-hand calcium-binding protein 3 (NECAB3) and is validated for Western blotting (WB) and immunocytochemistry/immunofluorescence (ICC/IF). The antibody is supplied as a 50 µL aliquot at a working strength of 1.0 mg/mL, formulated in phosphate buffered saline (without Mg²⁺ and Ca²⁺) at pH 7.4, supplemented with 150 mM NaCl, 0.02% sodium azide and 50% glycerol as a cryoprotectant. The host species is rabbit and the clonality is polyclonal, with the IgG fraction affinity-purified from antiserum by epitope-specific affinity chromatography using a synthetic peptide derived from the internal region of human NECAB3 as the immunogen. The conjugate is unconjugated, allowing researchers freedom to couple it to their preferred reporter or label, and the isotype is IgG. The reagent is reported to detect endogenous levels of total NECAB3 protein in human and mouse samples, making it suitable for cross-species studies. For best stability, the antibody is shipped at 4 °C; upon receipt it should be aliquoted and stored at –20 °C, with repeated freeze/thaw cycles avoided to preserve binding activity. The product is intended for research use only and is not for diagnostic or therapeutic applications.