Anti-NDUFV3 Antibody

Raised in rabbit against the human NADH:ubiquinone oxidoreductase core subunit V3, this unconjugated polyclonal antiserum from Antibodies.com is supplied as 100 µl of liquid reagent intended for Western Blot detection of endogenous NDUFV3 protein. The target is the approximately 10 kDa accessory flavoprotein of mitochondrial respiratory chain Complex I (NADH dehydrogenase; also called CI-9kD or NADH-ubiquinone oxidoreductase 9 kDa subunit), a nuclear-encoded subunit mapped to chromosome 21q22.3 (UniProt P56181, HGNC:7719, Entrez gene 4731) and embedded in the mitochondrial inner membrane, where more than 40 Complex I subunits cooperate to catalyze the rotenone-sensitive oxidation of NADH and reduction of ubiquinone. Researchers investigating Complex I biogenesis, oxidative phosphorylation, and respiratory chain deficiency use this reagent to probe NDUFV3 expression in human cell and tissue lysates resolved by SDS-PAGE and detected by immunoblotting. The rabbit polyclonal IgG reacts with the human antigen and supports studies of mitochondrial disease, Leigh syndrome, and isolated Complex I deficiency, where defects in any single subunit can destabilize the holoenzyme and impair electron transfer. Aliquot the antiserum on arrival and store frozen to limit repeated freeze-thaw cycles, or refrigerate for short-term use, in order to preserve binding activity across multiple Western Blot experiments in research use only workflows.