Anti-NDUFS8 Antibody

Sourced through the Antibodies.com catalogue, this rabbit polyclonal reagent is raised against the NDUFS8 subunit of mitochondrial respiratory Complex I (also known as CI-23kD, Complex I-23kD, NADH-ubiquinone oxidoreductase 23 kDa subunit, or TYKY subunit). The antibody is supplied as an unconjugated, antigen-affinity purified preparation in a 100 µl vial, formulated in 0.1 M Tris-buffered saline at pH 7.0 containing 20% glycerol with 0.01% thimerosal added as a preservative. The immunogen corresponds to a recombinant protein fragment spanning a region within amino acids 1–208 of human NDUFS8, and the reagent is validated for Western blot (WB) and Immunohistochemistry (IHC) applications on human samples. Recommended handling is straightforward: the product is shipped at 4 °C, and upon arrival researchers should aliquot and store it at –20 °C, avoiding repeated freeze/thaw cycles to preserve activity. In Western blot, the supplier illustrates detection using 30 µg of whole-cell lysate from Hep G2 cells on a 12% SDS-PAGE gel with the antibody diluted at 1:1000, providing a useful working benchmark. The polyclonal nature of the reagent, combined with its unconjugated format, makes it suitable for downstream detection with a secondary antibody of choice, while its specificity for NDUFS8 makes it appropriate for studies of mitochondrial electron transport chain function, oxidative phosphorylation, and related metabolic disease research. For research use only; not intended for diagnostic or therapeutic applications.