Anti-NDUFS7 Antibody

NDUFS7 (NADH-ubiquinone oxidoreductase iron-sulfur protein 7) is a core catalytic subunit of mitochondrial respiratory chain complex I, anchoring the interaction with ubiquinone and contributing to proton translocation during oxidative phosphorylation. Defects in NDUFS7 cause Leigh syndrome and mitochondrial complex I deficiency, making it a recurring target in studies of neurodegeneration, metabolic disease, and mitochondrial bioenergetics.

This rabbit polyclonal IgG reagent from Antibodies.com is supplied at 200 µl in an unconjugated format, giving researchers flexibility to pair it with their preferred labeled secondary detection system. It is validated for western blot (WB), immunohistochemistry (IHC), and immunofluorescence (IF) workflows and reports reactivity with human, mouse, and rat samples, supporting detection of endogenous NDUFS7 across cultured cells, primary cultures, and tissue sections.

The NDUFS7 protein migrates at approximately 20 kDa on SDS-PAGE, consistent with its mitochondrial localization and its designation as the complex I 20 kDa subunit (CI-20kD). The unconjugated IgG format is compatible with both fluorescence and chromogenic detection, and the 200 µl volume supports multiple experimental runs at typical working dilutions. Researchers commonly use this reagent to probe complex I assembly, mitochondrial dysfunction models, and changes in respiratory chain subunit expression under oxidative or pharmacological challenge. Storage conditions, working dilution, and species-appropriate controls should be confirmed from the current lot documentation before use.