Anti-NDUFS5 (E89) Antibody

Raised in rabbit against an epitope mapping to the E89 region of NDUFS5, this unconjugated polyclonal antibody targets the iron-sulfur cluster subunit of mitochondrial respiratory chain complex I (NADH:ubiquinone oxidoreductase). Distributed by Antibodies.com at 1 mg/ml in phosphate buffered saline containing 0.05% sodium azide at approximately pH 7.2, the reagent is supplied as a 100 µl vial ready for routine immunolabelling workflows.

Validated for Western blot and immunohistochemistry, the antibody cross-reacts with NDUFS5 from human, mouse, and rat samples, supporting studies of oxidative phosphorylation, mitochondrial biogenesis, and metabolic disease models. Polyclonal recognition of the conserved E89 region allows binding to full-length and partially processed forms of the subunit under denaturing or fixed conditions, while the unconjugated format lets researchers pair the primary with HRP, biotin, fluorophore, or alkaline phosphatase secondary reagents for chromogenic or fluorescence detection.

Store refrigerated; long-term storage at -20°C in working aliquots limits repeated freeze-thaw exposure of the antibody solution. Aliquotting on first use helps preserve the activity of the stock for ongoing mitochondrial and cell biology projects.