Anti-NDUFS4 Antibody

This unconjugated rabbit polyclonal primary antibody targets NDUFS4, the nuclear-encoded mitochondrial Complex I subunit (NADH:Ubiquinone Oxidoreductase Core Subunit S4), and is supplied by Antibodies.com for Western blot and immunofluorescence workflows.

Raised in rabbit, the reagent is provided as 100 µl of polyclonal antiserum validated for human, mouse, and rat samples. Researchers commonly apply it to studies of Complex I biogenesis, mitochondrial respiratory chain dysfunction in neurodegeneration, and Leigh syndrome-associated mitochondrial disease models.

For Western blot, the antibody can be used on whole-cell or mitochondrial-enriched lysates to detect the mature, matrix-processed form of NDUFS4 that accumulates upon proper Complex I assembly. In immunofluorescence, it labels mitochondrial networks in cultured mammalian cells, co-localizing with markers such as TOMM20 or COX IV.

Refrigerate the vial upon arrival and aliquot the stock to preserve activity across multiple experiments. Avoid repeated freeze-thaw cycles. The unconjugated format allows pairing with custom secondary antibodies or affinity-purified IgG fractions for multiplexed imaging and quantitative blotting.