Anti-NDUFS2 Antibody

From the Antibodies.com catalogue, this primary antibody is a rabbit polyclonal reagent raised against NDUFS2, the 49 kDa iron-sulfur subunit (CI-49kD) of human mitochondrial NADH-ubiquinone oxidoreductase (Complex I). It is supplied as an unconjugated IgG in a 100 µl volume, purified by antigen-affinity chromatography and formulated in 0.1 M Tris-buffered saline (pH 7.0) containing 20% glycerol, with 0.01% thimerosal added as a preservative. The immunogen corresponds to a recombinant protein fragment spanning amino acids 200–400 of NDUFS2, and the antibody detects endogenous levels of total NDUFS2 protein. Validated applications include Western blot (WB) and immunohistochemistry (IHC), making the reagent suitable for studies of mitochondrial respiratory chain assembly, oxidative phosphorylation, and related metabolic research. Reactivity is reported for human samples, while cross-reactivity with other species has not been characterized. The reagent is supplied in liquid format, ready for use in standard immunoassays following the recommended storage conditions: it is shipped at 4 °C and, upon delivery, should be aliquoted and stored at –20 °C, with repeated freeze/thaw cycles avoided to preserve antibody integrity. For research use only, this polyclonal offers a reliable option for laboratories investigating Complex I subunit expression in human tissues and cell models.