Anti-NDUFB9 Antibody

The NDUFB9 protein is a small accessory subunit of mitochondrial Complex I (NADH:ubiquinone oxidoreductase), the first enzyme of the electron transport chain, and is frequently targeted in studies of oxidative phosphorylation, mitochondrial respiratory function, and metabolic disease. This polyclonal reagent, raised in rabbit and supplied by Antibodies.com as an unconjugated primary antibody, recognizes the human NDUFB9 target and is validated for Western blot and immunohistochemistry workflows, supporting detection of endogenous protein in cultured human cells and fixed tissue sections.

Provided as a 100 µL liquid aliquot, the antibody is suited to standard immunoblotting and IHC protocols and is often used as a marker when probing Complex I subunit composition, mitochondrial content, or assembly defects. Because NDUFB9 expression can vary with mitochondrial biogenesis and disease state, the reagent is commonly paired with reference markers such as VDAC1, COX IV, TOM20, or other Complex I subunits (for example NDUFA9, NDUFS1, or NDUFS3) when characterizing respiratory chain integrity or Complex I deficiency models. Storage at 4 °C is appropriate for short-term use, and aliquoting is recommended to limit freeze-thaw cycles and preserve antibody activity over longer periods.

Researchers typically apply this antibody to human cell lines, primary cultures, and tissue sections, and the polyclonal format provides epitope diversity that can be advantageous for detecting NDUFB9 across both denatured (Western blot) and fixed-tissue (IHC) conditions. As with any primary antibody from Antibodies.com, lot-to-lot validation data should be reviewed before scaling up experimental use, particularly when interpreting relative band intensity or subcellular localization in patient-derived or disease model samples.