Anti-NDUFB9 Antibody

This rabbit polyclonal antibody targets NDUFB9, an accessory subunit of mitochondrial Complex I (NADH:ubiquinone oxidoreductase) embedded in the inner mitochondrial membrane. NDUFB9 contributes to the proper assembly and stability of the ~1 MDa respiratory Complex I, and it has been studied in the context of mitochondrial disease biology, including Complex I deficiency and Leigh syndrome research, where loss of the subunit destabilizes holoenzyme integrity and impairs electron entry into the respiratory chain.

Provided as 100 µl of unconjugated antiserum raised in rabbit, the reagent is validated for western blot, immunohistochemistry, and ELISA applications and reacts with human samples. For western blot, researchers commonly resolve mitochondrial or whole-cell lysates near the 22 kDa mature NDUFB9 polypeptide. For IHC, the antibody supports localization studies in fixed tissue sections, and the unconjugated format permits downstream pairing with user-selected reporter-conjugated secondary antibodies or custom signal-amplification workflows. Storage at 4°C short term and -20°C long term is recommended to preserve activity.

Manufactured and quality-controlled by Antibodies.com, each lot is released with application-specific validation data so that mitochondrial bioenergetics laboratories, oxidative phosphorylation researchers, and translational groups investigating nuclear-encoded Complex I subunit defects can integrate the antibody into established immunodetection pipelines with confidence.