Anti-NDUFA8 Antibody

Raised in rabbit and affinity-purified by epitope-specific immunogen chromatography, this polyclonal reagent from Antibodies.com targets the NDUFA8 subunit of mitochondrial complex I and is supplied at a working concentration of 1.0 mg/mL in a 50 µL volume of phosphate-buffered saline (pH 7.4, 150 mM NaCl) containing 0.02% sodium azide and 50% glycerol as a cryoprotectant. The unconjugated antibody detects endogenous levels of total NDUFA8 protein and has been validated for Western blot (WB) and immunohistochemistry (IHC) applications on human and mouse samples, making it a useful tool for studies in mitochondrial bioenergetics, oxidative phosphorylation, and complex I assembly research. The immunogen is a synthesized peptide derived from the internal region of human NDUFA8, while the formulation is built on rabbit IgG as the native isotype, ensuring compatibility with standard anti-rabbit secondary reagents. For routine handling, the product is shipped at 4 °C and should be aliquoted upon arrival and stored at –20 °C, with repeated freeze/thaw cycles avoided to preserve binding activity and reagent stability over multiple experiments. Provided at research-use-only grade by Antibodies.com and dispatched from St. Louis, MO, this affinity-purified polyclonal offers laboratories a reliable probe for characterizing NDUFA8 expression across cell and tissue lysates.