Anti-NDC80 Antibody - Identical to Abcam (ab186839)

A rabbit polyclonal antibody directed against NDC80, also designated HEC1/HEC, is offered by Antibodies.com as a primary antibody reagent. Raised in rabbit and of the IgG isotype, the unconjugated antibody is validated for Western blot (WB) applications and is reactive with human and mouse samples.

The immunogen is a recombinant fusion protein containing a sequence corresponding to amino acids 343-642 of human HEC1/HEC1/NDC80 (NP_006092.1). The reagent is supplied as a liquid in Phosphate Buffered Saline at pH 7.3, containing 50% glycerol and 0.02% sodium azide, and is purified by affinity purification. The target protein has a reported molecular weight of 80 kDa.

For Western blot, the manufacturer recommends a working dilution range of 1:500-1:2,000. The product is shipped at 4°C and, upon receipt, should be aliquoted and stored at -20°C, with freeze/thaw cycles avoided. One scientific validation image accompanies the product.