Anti-NCSTN Antibody

Raised in rabbit and affinity-purified as an unconjugated IgG polyclonal preparation, this primary antibody from Antibodies.com targets the Nicastrin (NCSTN) subunit of the gamma-secretase complex for western blot applications. The reagent is supplied as a 100 µL liquid aliquot and is validated for detection of endogenous Nicastrin in human and rat samples, where the mature glycoprotein migrates at approximately 110 kDa. Nicastrin acts as a stabilizing scaffold within gamma-secretase, which mediates intramembrane cleavage of substrates such as the amyloid precursor protein and Notch receptors, making the antibody a useful tool for studying amyloidogenic processing and Notch-dependent signaling. It is also relevant to dermatology research, because heterozygous loss-of-function NCSTN variants cause familial hidradenitis suppurativa, and the polyclonal format increases the chance of recognizing multiple epitopes across the Nicastrin extracellular domain. The antibody is provided in a PBS-based buffer with 50% glycerol and 0.01% thiomersal and should be stored at -20 degrees C with protection from light to preserve activity across multiple freeze-thaw cycles.