Anti-NCK1 Antibody

This rabbit polyclonal antibody from Antibodies.com targets NCK1, a non-catalytic SH2/SH3 adaptor protein that links activated receptor tyrosine kinases to downstream effectors controlling actin cytoskeleton remodeling, cell spreading, and migration. The reagent is supplied as 100 µl of unconjugated antibody at 1 mg/ml in PBS with 0.1% Sodium Azide and 50% Glycerol, shipped as a single-use vial compatible with western blot (WB) and immunohistochemistry (IHC) workflows.

Validation has been confirmed on samples of human, mouse, and rat origin, covering the most common laboratory model systems. NCK1, also referenced as Nck or Nck-alpha, couples signaling from receptors such as Eph, PDGFR, and the T cell receptor to the N-WASP/WAVE2 machinery, so researchers studying tyrosine phosphorylation cascades, focal adhesion turnover, and immune synapse formation commonly rely on this reagent to detect the endogenous adaptor.

Handling notes: aliquot upon first use to minimize freeze-thaw cycles and store the glycerol-containing stock at -20°C; the sodium azide preservative permits short-term benchtop handling. Applications include western blot to resolve the adaptor near its expected molecular weight (~43 kDa) and IHC on fixed tissue sections to map NCK1 distribution.