Anti-NCK1 Antibody

This Primary Antibodies reagent from Antibodies.com is a rabbit polyclonal antibody raised against NCK1, supplied as an unconjugated IgG. It is validated for Western blot, with the catalogue entry additionally listing immunofluorescence, and manufacturer data also supporting immunohistochemistry. The reagent shows reactivity with human, mouse, and rat samples, and is affinity-purified from rabbit host serum.

Manufacturer documentation describes a liquid formulation in phosphate-buffered saline containing 50% glycerol and 0.02% sodium azide, shipped at 4°C and intended for storage at -20°C after aliquoting. The immunogen is a recombinant fusion protein corresponding to amino acids 58-377 of human NCK1 (NCBI reference NP_006144.1), and the target protein has a reported molecular weight of 43 kDa. Recommended working dilutions are 1:500-1:2,000 for Western blot and 1:50-1:100 for immunohistochemistry.