Anti-NCF4 Antibody

Offered by Antibodies.com as a Primary Antibody, this rabbit polyclonal reagent recognises NCF4, also referred to as p40-phox. It is supplied as a liquid at lot-specific concentration, is unconjugated, and has an IgG isotype. The antibody was raised against a synthetic phosphorylated peptide around T154 of human NCF4/p40-phox (NP_000622.2), with the immunogen sequence PRTRK, and was purified by affinity chromatography.

The reagent is formulated in Phosphate Buffered Saline at pH 7.3, containing 50% glycerol and 0.01% thiomersal. It is shipped at 4°C and should be aliquoted and stored at -20°C, with freeze/thaw cycles avoided. The manufacturer reports the target protein's molecular weight as 40 kDa and recommends a working dilution of 1:500–1:1,000 for Western blot.

The antibody is validated by the manufacturer for Western blot and is reactive with Human, Mouse, and Rat samples; the Antibodies.com product listing additionally lists IHC and IF among its applications. One scientific validation image is referenced by the manufacturer.