Anti-NCF1 Antibody

NCF1 (Neutrophil Cytosolic Factor 1), also called p47phox, is a cytosolic organizer subunit of the phagocyte NADPH oxidase complex. During the respiratory burst, phosphorylated p47phox translocates to the membrane and helps assemble the active oxidase that generates superoxide. This unconjugated Rabbit polyclonal reagent from Antibodies.com is supplied as 100 µL of liquid, intended for Western blot and immunohistochemistry on human, mouse, and rat specimens. The antibody binds the NCF1 target in cell lysates and tissue sections, supporting studies of chronic granulomatous disease, neutrophil oxidative burst signaling, and innate immune regulation. Polyclonal recognition of multiple epitopes is useful when probing phosphorylation-driven conformational changes in p47phox, or when working with formalin-fixed paraffin-embedded tissue where epitope masking is common. Typical uses include characterizing NADPH oxidase assembly, profiling reactive oxygen species pathways in inflammatory disease models, and mapping neutrophil dysfunction in primary immunodeficiencies. Store refrigerated at 2-8 degrees C in single-use aliquots to limit freeze-thaw cycles, and protect from light to preserve the unconjugated preparation.