Anti-NCAM2 Antibody

A polyclonal affinity-purified reagent raised in rabbit against Neural Cell Adhesion Molecule 2 (NCAM2), also known as OCAM, an immunoglobulin superfamily glycoprotein that mediates homophilic cell-cell adhesion in the developing and adult nervous system. Antibodies.com supplies the unconjugated IgG in a 100 µl vial, validated for Western blot detection of endogenous NCAM2 (~92 kDa) in human, mouse, and rat samples.

NCAM2 carries five immunoglobulin modules and two fibronectin type 3 homology modules in its ectodomain and exists as both transmembrane and GPI-anchored isoforms. The protein is most abundant in brain, where it concentrates in dendritic spines and supports neurite outgrowth, axon fasciculation, synaptogenesis, and zone-to-zone projection of primary olfactory axons to glomeruli in the main olfactory bulb. Because the polyclonal response targets multiple epitopes on the NCAM2 ectodomain, the antibody recognises variant isoforms or partially proteolysed fragments, and it does not cross-react with the paralog NCAM1.

The unconjugated IgG is paired in Western blot with a secondary reagent such as HRP-conjugated anti-rabbit IgG for chemiluminescent detection. Researchers have used comparable Antibodies.com reagents to characterise NCAM2 expression in hippocampal and cortical lysates, monitor synaptic adhesion-molecule changes in plasticity assays, and explore associations in schizophrenia and bipolar disorder models. Store refrigerated and protect from light; avoid repeated freeze-thaw cycles to preserve antibody performance.