Anti-NCAM1 Antibody

This unconjugated rabbit polyclonal antibody from Antibodies.com targets neural cell adhesion molecule 1 (NCAM1), a heavily glycosylated transmembrane glycoprotein that mediates homophilic cell-cell binding, neurite outgrowth, and synaptic plasticity in the nervous system. The reagent is supplied as 100 µl of a 1 mg/ml stock in phosphate buffered saline at approximately pH 7.3, with 0.05% sodium azide added as a preservative. Clonality is polyclonal, the host is rabbit, and the conjugate is unconjugated, allowing researchers to pair the antibody with HRP-, fluorophore-, or biotin-tagged secondaries of their own choosing. Validated reactivity covers human, rat, and mouse samples, supporting comparative studies in neurodevelopmental biology and NK-cell immunology, since NCAM1 is also known as the CD56 surface marker on natural killer cells. The antibody is validated for western blot application, where the characteristic NCAM1 expression profile, often resolved as multiple bands near 120, 140, and 180 kDa reflecting the three principal isoforms and variable polysialylation, can be visualized by chemiluminescence or fluorescence imaging. For handling, aliquot the reagent upon arrival to minimize freeze-thaw cycles, store refrigerated for routine use, and keep frozen at -20 °C for long-term preservation.