Anti-NBR1 Antibody

Selective-autophagy cargo receptors sit at the center of how cells dispose of damaged organelles, misfolded proteins, and intracellular pathogens, and Antibodies.com supplies this unconjugated rabbit polyclonal reagent raised against NBR1 to help researchers profile those flux pathways on Western blot. The antibody recognizes native NBR1, including its PB1, ZZ-type zinc finger, and ubiquitin-associated (UBA) domain architecture, supporting detection of the long isoform in whole-cell lysates prepared in RIPA buffer with protease inhibitors.

Beyond canonical aggrephagy and mitophagy work, NBR1 is also implicated in pexophagy of damaged peroxisomes and in xenophagy of cytosolic bacteria, and is increasingly used alongside p62/SQSTM1 and TAX1BP1 to dissect cargo selectivity. The polyclonal response is an asset in these studies because it tolerates minor sequence variation across the receptor's functional regions, resolving the endogenous band at the expected molecular weight when 20 to 40 µg of total protein is loaded per lane. Stripping and reprobing the same membrane for LC3, ubiquitin, and a loading control such as GAPDH or actin helps confirm receptor accumulation under nutrient stress, oxidative challenge, or proteasome inhibition.

Validated for human, mouse, and rat samples, this 200 µl IgG reagent is unconjugated and is therefore compatible with standard anti-rabbit IgG secondaries conjugated to HRP, IRDye, or Alexa Fluor reporters for chromogenic or fluorescent detection. Store refrigerated, and aliquot if repeated freeze-thaw cycles are anticipated. For research use only.