Anti-N / H / K-Ras Antibody

Offered through Antibodies.com as a primary polyclonal reagent, this affinity-purified reagent is raised in rabbit against a synthetic peptide derived from the N-terminal region of human N/H/K-Ras, enabling broad detection of endogenous NRAS, HRAS and KRAS proteins. Supplied as a 50 µl liquid at 1 mg/ml in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, the unconjugated antibody is validated for Western Blot, Immunohistochemistry and ELISA workflows and has been demonstrated on 293 human cells and L929 rat muscle cells in WB. Cross-reactivity covers Human, Mouse and Rat samples, making it suitable for comparative signalling studies across the standard laboratory species. The polyclonal host format increases the likelihood of recognising multiple epitopes on the Ras family members, while the affinity-chromatography purification using the epitope-specific immunogen helps to minimise background. For best stability the vial should be stored at -20 °C, where it remains valid for up to one year, with standard freeze/thaw aliquoting recommended once the reagent is in use. Researchers planning chromogenic or fluorescent detection can pair this unconjugated preparation with secondary antibodies of their choice, and the 50 µl pack size provides ample volume for typical Western blot and IHC titration experiments.