Anti-MYPT1 (R690) Antibody

Raised against a synthetic peptide corresponding to amino acids 671–720 of human MYPT1, this affinity-purified polyclonal reagent from Antibodies.com is supplied as a 50 µL vial of unconjugated rabbit IgG at 1 mg/ml in PBS with 0.05% sodium azide at approximately pH 7.2. The antibody detects endogenous PPP1R12A and is reactive with Human, Mouse, and Rat samples in both Western blot and immunohistochemistry workflows, with recommended WB dilutions of 1:500–1:1000 and IHC dilutions of 1:50–1:200 on paraffin-embedded sections. The same reagent is also referenced under catalogue A01743, and published IHC validation has been performed on paraffin-embedded human breast carcinoma tissue at a 1:100 dilution. Researchers use this reagent to probe RhoA/ROCK-dependent phosphorylation of myosin light chain phosphatase, vascular smooth muscle contractility, cytoskeletal remodeling during cell migration, and altered phosphatase activity in cardiovascular and tumor models. Aliquot upon first use, store refrigerated, and avoid repeated freeze-thaw cycles to preserve activity across multiple experiments.