Anti-MYOZ1 Antibody

The Antibodies.com polyclonal antibody reagent targets Myozenin 1, a ~32 kDa Z-disc–localized protein expressed predominantly in fast-twitch skeletal muscle, where it links alpha-actinin-2 and gamma-filamin (FLNC) at the sarcomere and tethers calcineurin signaling to the contractile apparatus. The MYOZ1 gene maps to chromosome 10q22.1-q22.2, and the antibody is raised in rabbit and purified as an unconjugated IgG supplied in a 50 µl vial at working dilutions suitable for Western blot and immunoprecipitation workflows using human, mouse, and rat samples.

Endogenous MYOZ1 is readily detected in C2C12 myotube lysates, adult tibialis anterior homogenates, and primary myoblast cultures undergoing myogenic differentiation, making this reagent useful for time-course studies of myofibrillogenesis and for probing how Z-disc complexes remodel under mechanical loading, stretch, or disuse atrophy. The antibody co-precipitates native Z-disc binding partners (alpha-actinin-2, FLNC, TCAP/telethonin, LDB3/ZASP) from skeletal muscle extracts, supporting interactome mapping by mass spectrometry. Distal arthrogryposis type 2B (DA2B, Sheldon-Hall syndrome) has been linked to MYOZ1 missense variants, and laboratories investigating this condition use the reagent to compare patient-derived myotubes against isogenic controls. Aliquoting upon receipt and storing at -20 °C helps preserve polyclonal reactivity across repeated freeze-thaw cycles, and the rabbit IgG isotype is compatible with standard anti-rabbit HRP, Alexa Fluor, and IRDye secondary reagents for chemiluminescent, fluorescent, or near-infrared detection on immunoblots.