Anti-Myosin light chain 2 Antibody

This rabbit polyclonal antibody from Antibodies.com targets myosin light chain 2 (MLC-2), a regulatory subunit of conventional myosin that governs actomyosin ATPase activity in cardiac and skeletal muscle. Raised in rabbit and affinity purified, the reagent is supplied unconjugated at 1 mg/ml in PBS containing 0.1% sodium azide and 50% glycerol, in a 100 µl vial. The antibody has been validated for western blot, immunohistochemistry, and immunofluorescence workflows, and it cross-reacts with mouse, rat, and human samples, making it well-matched for comparative studies across model systems and patient-derived tissue. Researchers investigating sarcomere assembly, cardiomyocyte contractility, hypertrophic signaling, and smooth muscle differentiation routinely use MLC-2 markers to distinguish atrial from ventricular identity (MLC-2a versus MLC-2v isoforms) and to assess remodeling after injury. Aliquot upon first thaw to limit freeze-thaw cycles and store at -20 °C in working aliquots; the glycerol content helps protect the protein during frozen storage. For multiplexing, the unconjugated format allows direct pairing with secondary antibodies of the researcher's choice, including HRP, Alexa Fluor, or biotin conjugates depending on the detection modality.