Anti-MYLK Antibody

Raised in rabbit against the myosin light chain kinase (MYLK) target, this unconjugated polyclonal reagent from Antibodies.com is supplied at 1 mg/ml in phosphate buffered saline (PBS) with 0.05% sodium azide at approximately pH 7.2. The 100 µl volume supports routine western blot workflows detecting the kinase in lysates prepared from human, mouse, and rat samples.

MYLK phosphorylates the regulatory light chain of myosin II, driving actomyosin cross-bridge cycling in smooth muscle and contributing to cytoskeletal tension, cell migration, and barrier function in non-muscle cells. The reagent is useful for studies of vascular smooth muscle contraction, endothelial permeability, asthmatic airway hyperresponsiveness, and cytoskeletal remodeling in cancer invasion models. Polyclonal reactivity broadens epitope coverage across the kinase region, an advantage when probing isoforms such as the short and long smMLCK variants generated by alternative transcriptional start sites.

Storage at 4°C is appropriate for short-term use; aliquoting the 1 mg/ml stock avoids repeated freeze-thaw cycles that can compromise antibody integrity. The unconjugated format allows the researcher to choose HRP, fluorescent, or biotin reporter chemistries matched to their chosen detection platform.