Anti-MYL9 Antibody

Raised in rabbits and affinity-purified against an immunizing synthetic peptide derived from human MYL9, this polyclonal reagent from Antibodies.com is supplied as an unconjugated antiserum at 1.0 mg/mL in a 50 µL aliquot. The host species is Rabbit, the clonality is polyclonal, and the immunoglobulin fraction was isolated by affinity purification using the immunogen, ensuring strong specificity for the endogenous total MYL9 protein (myosin regulatory light chain 9). It is validated for Western blotting (WB) and immunocytochemistry/immunofluorescence (ICC/IF), and reactivity has been confirmed in Human, Mouse and Rat samples, making it a versatile tool for smooth-muscle and cytoskeletal studies across mammalian models. The product is formulated in phosphate-buffered saline (without Mg²⁺ and Ca²⁺) at pH 7.4, containing 150 mM NaCl, 0.02% sodium azide and 50% glycerol as a cryoprotectant. It is shipped cold at 4°C and should be aliquoted upon receipt and stored at -20°C, with repeated freeze/thaw cycles avoided to preserve antibody integrity. Recommended working dilutions typically start in the 1:500–1:2,000 range for WB depending on the detection system, while ICC/IF applications are usually optimized at lower dilutions by the end user. Researchers investigating myosin light-chain phosphorylation, smooth-muscle contraction, vascular biology, or cytoskeletal remodelling will find this Antibodies.com reagent a reliable primary antibody for both routine western blots and fluorescence imaging workflows.