Anti-MYL3 Antibody

This unconjugated rabbit polyclonal antibody from Antibodies.com targets myosin light chain 3 (MYL3), the ventricular cardiac isoform that pairs with the essential light chain within the thick filament of the sarcomere. Supplied as 100 µl of a 1 mg/ml stock in PBS with 0.1% sodium azide and 50% glycerol, the reagent is validated for Western blot, immunohistochemistry, and immunofluorescence workflows on human, mouse, and rat samples. MYL3 is a well-characterized marker of ventricular cardiomyocyte identity and is implicated in hypertrophic cardiomyopathy, where inherited substitutions such as the A13T and R57H variants alter calcium binding and contractile behavior. The antibody is therefore widely applied in cardiac developmental biology, hypertrophy and heart-failure models, and characterization of iPSC-derived cardiomyocytes, where cross-reactivity across the three listed species supports comparative tissue studies. The polyclonal format recognizes multiple epitopes, improving tolerance for partial protein degradation in archival paraffin sections and helping preserve signal on mildly denatured lysates. As an unconjugated reagent, the antibody can be paired with custom secondary conjugates - HRP, biotin, Alexa Fluor, or alkaline phosphatase - to suit either chromogenic or fluorescence detection platforms.